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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualisering av den immunologiska Synapse av Dual Color Time-gated stimulerad emission Utarmning (Sted) nanoskopi
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Visualisering av den immunologiska Synapse av Dual Color Time-gated stimulerad emission Utarmning (Sted) nanoskopi

Article DOI: 10.3791/51100-v • 10:00 min • March 24th, 2014
March 24th, 2014 •
1, 1
1Center for Human Immunobiology, Texas Children's Hospital and Baylor College of Medicine

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Här illustrerar vi det protokoll för avbildning med tvåfärgad Sted nanoskopi den cytotoxiska immun synapsen av NK-celler rekapituleras på glas. Med denna metod får vi sub-100 nm upplösning på synapsproteiner och cytoskelettet.

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Immunologi naturliga mördarceller F-aktin immun synaps super-upplösning mikroskopi tvåfärgad stimulerad emission utarmning (Sted) mikroskopi
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