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Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle
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Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

Article DOI: 10.3791/51195-v • 10:52 min • August 21st, 2014
August 21st, 2014 •
1, 2,3, 4, 5, 6, 7,8, 9
1Stem Cell Research Center, Department of Bioengineering and Orthopedic Surgery, University of Pittsburgh, 2Department of Orthopedic Surgery, University of Pittsburgh, 3Nazarbayev University Research and Innovation System, Nazarbayev University, 4Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital and the Orthopaedic Hospital Research Center, University of California at Los Angeles, 5Department of Cell Biology, Erasmus MC Stem Cell Institute, 6OHSU Center for Regenerative Medicine, Oregon Health & Science University, 7Centre for Cardiovascular Science and MRC Centre for Regenerative Medicine, Queen's Medical Research Institute and University of Edinburgh, 8David Geffen School of Medicine and the Orthopaedic Hospital Research Center, University of California at Los Angeles, 9Stem Cell Research Center, Department of Orthopedic Surgery and McGowan Institute for Regenerative Medicine, University of Pittsburgh

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Resumo
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Blood vessels within human skeletal muscle harbor several multi-lineage precursor populations that are ideal for regenerative applications. This isolation method allows simultaneous purification of three multipotent precursor cell populations respectively from three structural layers of blood vessels: myogenic endothelial cells from intima, pericytes from media, and adventitial cells from adventitia.

Tags

Isolation Blood-vessel-derived Multipotent Precursors Human Skeletal Muscle Mesenchymal Stem/stromal Cells Native Identity Localization Retrospective Isolation Culture Fluorescence-activated Cell Sorting FACS Subpopulations Myogenic Endothelial Cells MECs Pericytes PCs Adventitial Cells ACs Structural Layers Blood Vessels Intima Media Adventitia Classic MSC Markers Mesodermal Developmental Potentials Isolation Protocol Selective Cell Surface Markers Human Muscle Biopsy Clinical Applications
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