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Measurement of Metabolic Rate in Drosophila using Respirometry
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Biologia
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JoVE Journal Biologia
Measurement of Metabolic Rate in Drosophila using Respirometry

Measurement of Metabolic Rate in Drosophila using Respirometry

DOI:
10.3791/51681-v

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04:31 min

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June 24, 2014

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, , ,
1Max Planck Research Group of Gene Expression and Signaling,Max Planck Institute for Biophysical Chemistry

Capítulos

  • 00:05Título
  • 00:57Respirometer and Measurement Chamber Preparation
  • 01:52Placing Flies into Respirometers and Performing the Experiment
  • 03:19Analysis
  • 03:50Results: Increased CO2 Production in Dystroglycan Mutant Flies
  • 04:12Conclusion

Summary

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Metabolic disorders are among one of the most common diseases in humans. The genetically tractable model organism D. melanogaster can be used to identify novel genes that regulate metabolism. This paper describes a relatively simple method which allows studying the metabolic rate in flies by measuring their CO2 production.

Tags

DrosophilaMetabolic RateRespirometryMetabolic DisordersSignaling PathwaysGenetic ScreensCO2 ProductionSubstrate OxidationEnergy ExpenditureMetabolic State

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