3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

Sheena Pinto; Hans-J&#252;rgen Stark; Iris Martin; Petra Boukamp; Bruno Kyewski

doi:10.3791/52614

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3D Organotípicas Co-cultura Modelo de Apoio medular Thymic Epithelial proliferação, diferenciação e Expression Promiscuous Gene

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

3D Organotípicas Co-cultura Modelo de Apoio medular Thymic Epithelial proliferação, diferenciação e Expression Promiscuous Gene

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10,613 Views

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06:47 min

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July 30, 2015

DOI:

10.3791/52614-v

DOI:

10.3791/52614-v

•

06:47 min

July 30, 2015

•

3 Views

Sheena Pinto*¹, Hans-Jürgen Stark*², Iris Martin², Petra Boukamp², Bruno Kyewski¹

¹Division of Developmental Immunology,German Cancer Research Center (DKFZ), ²Genetics of Skin Carcinogenesis,German Cancer Research Center (DKFZ)

Summary

Studying medullary thymic epithelial cells in vitro has been largely unsuccessful, as current 2D culture systems do not mimic the in vivo scenario. The 3D culture system described herein - a modified skin organotypic culture model - has proven superior in recapitulating mTEC proliferation, differentiation and maintenance of promiscuous gene expression.