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Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Article DOI: 10.3791/52801-v • 07:29 min • June 20th, 2015
June 20th, 2015 •
1,2, 1, 1,3, 3, 1,3
1Stem Cells & Cancer Group, Molecular Pathology Program, Spanish National Cancer Research Center, 2Institute for Research in Biomedicine (IRB Barcelona), 3Center for Stem Cells in Cancer & Ageing, Barts Cancer Institute, Queen Mary University of London

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Pancreatic cancer stem cells (CSCs) can be expanded in vitro using the anchorage-independent sphere culture technique, which represents a powerful tool to study CSC biology and can serve as the first step to develop novel CSC-targeting therapies. Here the methodology for expanding, analyzing and targeting of pancreatic CSCs is provided.

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Pancreatic Cancer Cancer Stem Cells Treatment Strategies Tumor Initiation Metastasis Resistance To Radio- And Chemotherapy Culturing Primary Human Pancreatic CSCs Tumor Spheres Serum-free Conditions Non-adherent Conditions Enriched For CSCs Differentiated Progenies Percentage Of CSCs Size Of Tumor Spheres Number Of Tumor Spheres CSC Activity Metformin Pluripotency-associated Genes/surface Markers In Vivo Tumorigenicity Preclinical Development Mice Bearing Established Tumors Prolonged Survival Clinical Studies
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