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JoVE Journal Developmental Biology
Tredimensionell Kvantifiering av Dendritutskotten från Pyramidal nervceller Härstammar från Human inducerade pluripotenta stamceller
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Tredimensionell Kvantifiering av Dendritutskotten från Pyramidal nervceller Härstammar från Human inducerade pluripotenta stamceller

Article DOI: 10.3791/53197-v • 10:18 min • October 10th, 2015
October 10th, 2015 •
1,2,3, 4, 1,2,3, 5, 1,2,3, 1,2,3
1Human Genetics and Cognitive Functions, Institut Pasteur, 2CNRS URA 2182 'Genes, synapses and cognition', Institut Pasteur, 3Human Genetics and Cognitive Functions, Université Paris Diderot, Sorbonne Paris Cité, 4Plateforme d' Imagerie Dynamique, Imagopole, CiTech, Institut Pasteur, 5Neuroplasticity and Therapeutics, CECS, I-STEM, AFM, Evry

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Dendritutskotten av pyramidala nervceller är platserna för de flesta excitatoriska synapser i däggdjurs hjärnbarken. Denna metod beskriver en 3D kvantitativ analys av rygg morfologier i humana kortikala pyramidala glutamaterga neuroner som härrör från inducerade pluripotenta stamceller.

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Utvecklingsbiologi Human iPSC-härledda neuroner pyramidala nervceller dendritiska ryggraden mognad ryggrad imaging 3D kvantifiering konfokalmikroskopi
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