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JoVE Journal Biochemistry

LC-MS / MS 분석을 이용하여 핵 공동 인자와 상호 작용 단백질의 높은 처리량 식별 단백질 제조 방법

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Journal (Biochemistry)

A Method for Measuring RNA N⁶-methyladenosine Modifications in Cells and Tissues

Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

Journal (Biochemistry)

Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

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LC-MS / MS 분석을 이용하여 핵 공동 인자와 상호 작용 단백질의 높은 처리량 식별 단백질 제조 방법

Article DOI: 10.3791/55077-v • 05:43 min • January 24th, 2017

January 24th, 2017 •

Megumi Tsuchiya*¹, M. Rezaul Karim*², Taro Matsumoto³, Hidesato Ogawa¹, Hiroaki Taniguchi^3,4,5

¹Graduate School of Frontier Biosciences, Osaka University, ²Department of Biotechnology and Genetic Engineering, Jahangirnagar University, ³Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University, ⁴Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, ⁵Graduate School of Life and Medical Sciences, Doshisha University

* These authors contributed equally

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우리는 LC-MS / MS 시스템을 사용 coregulatory 상호 작용 단백질의 정제 방법을 확립 하였다.

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생화학 문제 119 단백질 상호 작용 LC-MS / MS 단백질 정제 면역 핵 수용체 보조 인자 전사 크로 마틴 리모델링 인자 단백질 복합체

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