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JoVE Journal Developmental Biology
La diferenciación eficiente de pluripotentes células madre a NKX6-1
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La diferenciación eficiente de pluripotentes células madre a NKX6-1

Article DOI: 10.3791/55265-v • 09:23 min • March 7th, 2017
March 7th, 2017 •
1,2,3, 1,2,3
1Toronto General Research Institute, University Health Network, 2McEwen Centre for Regenerative Medicine, University Health Network, 3Department of Physiology, University of Toronto

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Aquí se describe un protocolo de 4 etapas para diferenciar células madre embrionarias humanas para NKX6-1 + progenitoras pancreáticas in vitro. Este protocolo se puede aplicar a una variedad de líneas de células madre pluripotentes humanas.

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Biología del Desarrollo No. 121 la diabetes el páncreas las células madre embrionarias humanas humanas pluripotentes células madre la medicina regenerativa pancreáticos Progenitores las células beta Diferenciación Citometría de Flujo
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