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Prøveforberedelse til massespektrometri-baseret identifikation af RNA-bindende regioner
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Prøveforberedelse til massespektrometri-baseret identifikation af RNA-bindende regioner

Article DOI: 10.3791/56004-v • 10:52 min • September 28th, 2017
September 28th, 2017 •
1,4, 1,2, 1,3, 1,3, 1,2
1Epigenetics Institute, University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine

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Vi beskriver en protokol for at identificere RNA-bindende proteiner og kortlægge deres RNA-bindende regioner i levende celler ved hjælp af UV-medieret photocrosslinking og massespektrometri.

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Biokemi sag 127 Protein-RNA interaktioner RNA-bindende domæne RNA-bindende region massespektrometri noncoding RNA'er UV crosslinking
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