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A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
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A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s

Article DOI: 10.3791/56312-v • 07:38 min • September 25th, 2017
September 25th, 2017 •
1,2, 2, 2
1Department of Pharmacology, University of Minnesota, 2Masonic Cancer Center, University of Minnesota

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α-hydroxylation of carcinogenic nitrosamines by cytochrome P450s is the accepted metabolic pathway that produces DNA-damaging intermediates, which cause mutations. However, new data indicates further oxidation to nitrosamides can occur. We describe a general method for detecting nitrosamides produced from in vitro cytochrome P450-catalyzed metabolism of nitrosamines.

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Nitrosamide Formation In Vitro Metabolism Nitrosamines Cytochrome P450s Toxicology Field Carcinogenesis Mass Spectrometer Detection Limits Nitrosamine Metabolism Assays Round Bottom Flask Magnetic Stir Bar Oven Nitrogen Gas Norcotinine Acetic Anhydride Acetic Acid Ice Water Bath Sodium Nitrite Quench The Reaction Separatory Funnel DCM (dichloromethane) Aqueous Layer
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