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JoVE Journal Neuroscience
Visualisatie van Thalamocortical Axon vertakking en de Synapse formatie in Organotypic Cocultures
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Visualisatie van Thalamocortical Axon vertakking en de Synapse formatie in Organotypic Cocultures

Article DOI: 10.3791/56553-v • 06:16 min • March 28th, 2018
March 28th, 2018 •
1,2, 2
1Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University

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Dit protocol beschrijft een methode voor gelijktijdige beeldvorming van thalamocortical axon vertakking en de synapse formatie in organotypic cocultures van de thalamus en cortex cerebri. Individuele thalamocortical axonen en hun presynaptische terminals worden gevisualiseerd door een eencellige electroporation techniek met DsRed en synaptophysin van GFP-gelabeld.

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Neurowetenschappen kwestie 133 segment cultuur hersenschors thalamus synaptogenese axonale arborization corticale ontwikkelen time-laps imaging
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