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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualisere aktin og mikrotubulus Cytoskeletons på B-celle immun Synapse ved hjælp af stimuleret Emission udtynding (STED) mikroskopi
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Visualisere aktin og mikrotubulus Cytoskeletons på B-celle immun Synapse ved hjælp af stimuleret Emission udtynding (STED) mikroskopi

Article DOI: 10.3791/57028-v • 11:00 min • April 9th, 2018
April 9th, 2018 •
1, 1, 1
1Department of Microbiology and Immunology, University of British Columbia

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Vi præsenterer en protokol for at bruge STED mikroskopi til samtidigt billede actin strukturer, mikrotubuli og mikrotubulus plus-end bindende proteiner i B-celler, der har spredt på coverslips belagt med antistoffer til B-celle receptoren, en model for den indledende fase af immun synapse dannelse.

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Immunologi og infektion spørgsmål 134 immunologi B-celler immun synapse actin mikrotubuli organisering af mikrotubulus center (MTOC) plus-ende tracking proteiner super-resolution mikroskopi stimuleret emission udtynding (STED) mikroskopi
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