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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering av Peritoneum-derived mastceller och deras funktionella karakterisering med Ca2 +-imaging och degranulering analyser
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Isolering av Peritoneum-derived mastceller och deras funktionella karakterisering med Ca2 +-imaging och degranulering analyser

Article DOI: 10.3791/57222-v • 11:31 min • July 4th, 2018
July 4th, 2018 •
1, 1, 1, 1
1Institute of Pharmacology, Ruprecht-Karls Heidelberg University

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Här presenterar vi ett protokoll för att isolera och odla murina peritoneal mastceller. Vi beskriver också två protokoll för deras funktionell karakterisering: en fluorescerande avbildning av intracellulära gratis Ca2 + koncentration och en degranulering analys baserat på kolorimetriska kvantifiering av de utgivna β-hexosaminidase.

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Immunologi och infektion fråga 137 Peritoneal mastceller murina mastceller intracellulära fritt kalciumkoncentration fluorescens imaging degranulering medlaren release beta-hexosaminidase
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