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JoVE Journal Developmental Biology
Live Cell Imaging della segregazione cromosomica durante la mitosi
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Live Cell Imaging della segregazione cromosomica durante la mitosi

Article DOI: 10.3791/57389-v • 06:39 min • March 14th, 2018
March 14th, 2018 •
1, 2, 1
1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research, US Food and Drug Administration

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Questo protocollo descrive un metodo facile e conveniente per etichettare e visualizzare live cromosomi nelle cellule mitotiche utilizzando etichetta Histone2B-GFP BacMam 2.0 e un sistema di microscopia confocal di filatura disco.

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Biologia dello sviluppo problema 133 segregazione del cromosoma controllo del ciclo cellulare mandrino Assemblea checkpoint live cell imaging nocodazole fibroblasti embrionali del mouse proteina fosfatasi 2A Histone2B-GFP BacMam 2.0
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