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Journal / Developmental Biology / 細胞分裂時の染色体分配の生きているセルイメージ投射…
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JoVE Journal Developmental Biology
細胞分裂時の染色体分配の生きているセルイメージ投射
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細胞分裂時の染色体分配の生きているセルイメージ投射

Article DOI: 10.3791/57389-v • 06:39 min • March 14th, 2018
March 14th, 2018 •
1, 2, 1
1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research, US Food and Drug Administration

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このプロトコルでは、ラベルおよび Histone2B GFP BacMam 2.0 ラベルおよび回転のディスク共焦点顕微鏡システム使用して分裂期の細胞で生きている染色体の視覚化に簡単で便利な方法について説明します。

Tags

発生生物学、問題 133、染色体、細胞周期制御、スピンドル集合チェックポイント、ライブセル イメージング、ノコダゾール、マウス胚性線維芽細胞、タンパク質脱燐酸化酵素 2 a Histone2B GFP BacMam 2.0
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