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JoVE Journal Genetics
Lentiviral medieret produktion af Transgene mus
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Lentiviral medieret produktion af Transgene mus: en enkel og meget effektiv metode til direkte undersøgelse af grundlæggerne

Article DOI: 10.3791/57609-v • 10:21 min • October 7th, 2018
October 7th, 2018 •
1,2, 1, 1, 1
1UPMC Univ Paris 06, INSERM U1127, CNRS UMR 7225, Institut du Cerveau et de la Moelle épinière, ICM, Sorbonne Universités, 2UPMC Univ Paris 06, INSERM UMRS1166, Institute of Cardiometabolism and Nutrition, Sorbonne Universités

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Vi præsenterer her, en protokol for at fremme transgen integration og produktion af grundlægger Transgene mus med høj effektivitet ved en simpel indsprøjtning af en lentiviral vektor i perivitelline rum af et befrugtet oocyt.

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Genetik spørgsmål 140 Lentiviral vektorer Transgene mus transduktion af befrugtede oocytter overførsel af Integrativ gener injektion i perivitelline plads allestedsnærværende initiativtagere celle specifikke enhancer
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