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JoVE Journal Developmental Biology
Isolering af menneskelige endometrie stromale celler til In vitro- Decidualization
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Isolering af menneskelige endometrie stromale celler til In vitro- Decidualization

Article DOI: 10.3791/57684-v • 14:55 min • September 1st, 2018
September 1st, 2018 •
*1, *1, 1, 1
1Department of Obstetrics and Gynecology, Center for Reproductive Health Sciences, Washington University School of Medicine
* These authors contributed equally

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Denne undersøgelse præsenterer et godkendt og optimeret procedure for isolation og kultur af human endometrie stromale celler til at gennemføre i vitro decidualization analyse. Yderligere, denne undersøgelse giver en detaljeret metode til effektivt knockdown et bestemt gen ved hjælp af siRNAs i menneskelige endometrie stromale celler.

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Udviklingsmæssige biologi sag 139 udviklingsmæssige biologi progesteron østrogen cAMP primære kulturer menneskelige endometrie stromale celler siRNA Transfektion og in vitro- decidualization
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