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A Rapid Image-based Bacterial Virulence Assay Using Amoeba

Article DOI: 10.3791/57844-v • 05:22 min • June 27th, 2018
June 27th, 2018 •
1, 1,2
1Department of Physics and Astronomy, University of California, 2Department of Molecular Biology and Biochemistry, University of California

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Here, we present a protocol to measure the virulence of planktonic or surface-attached bacteria using D. discoideum (amoeba) as a host. Virulence is measured over a period of 1 h and host killing is quantified using fluorescence microscopy and image analysis. We demonstrate this protocol using the bacterium P. aeruginosa.

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Bacterial Virulence Assay Amoeba Bacterial Pathogenesis Virulence Effectors Surface-attached Implantonic Cells Fast Quantitative Technique E.coli Strain BR GYP Plate High Humidity Box Amoeba Spores PS Medium Antibiotic Antimicotic Solution Pseudomonas Aeruginosa PSB Media
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