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Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
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Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Article DOI: 10.3791/57862-v • 11:08 min • June 19th, 2018
June 19th, 2018 •
*1,2, *3, 1, 1, 1, 2, 1,2, 1,2, 1,2
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children's Hospital and the Department of Pediatrics, The Ohio State University
* These authors contributed equally

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A non-labeled, non-radio-isotopic method for DNA polymerase proofreading and a DNA repair assay was developed by using high-resolution MALDI-TOF mass spectrometry and a single nucleotide extension strategy. The assay proved to be very specific, simple, rapid, and easy to perform for proofreading and repair patches shorter than 9-nucleotides.

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Proofreading DNA Repair Assay Single Nucleotide Extension MALDI-TOF Mass Spectrometry Analysis DNA Polymers Fidelity DNA Repair Technique Specific Simple Rapid Easy To Perform Mass Spectrometry Instrumentation Data Interpretation Visual Demonstration Clinical Applications Mutation And Deputation Commercially Developed Mass Spectrometry Primer And Template Preparation TG Mismatch Substrate P21 With T28 Dilute Incubate
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