Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / 体外和体内实验的位定向诱变-以大肠杆菌rna相互作用为例…
JoVE Journal
Biochemistry

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Biochemistry
体外和体内实验的位定向诱变-以大肠杆菌rna相互作用为例
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
Journal (Biochemistry)
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
Journal (Biochemistry)
Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
 
Click here for the English version

体外和体内实验的位定向诱变-以大肠杆菌rna相互作用为例

Article DOI: 10.3791/58996-v • 07:04 min • February 5th, 2019
February 5th, 2019 •
1, 1, 1
1Department of Biochemistry and Molecular Biology, University of Southern Denmark

Capítulos

Resumo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

位定向诱变是一种用于引入脱氧核糖核酸 (dna) 中的特定突变的技术。该协议描述了如何进行现场定向诱变与2步和3步聚合酶链反应 (pcr) 为基础的方法, 这是适用于任何 dna 片段的利益。

Tags

生物化学 第144期 诱变 诱变分析 位定向 emsa 西方印迹 rna-rna 相互作用 rna-蛋白质相互作用 寡核苷酸定向诱变 双质体
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter