Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / 使用声学纳米分配技术实现高通量DNA质粒多路复用和转染…
JoVE Journal
Genetics

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Genetics
使用声学纳米分配技术实现高通量DNA质粒多路复用和转染
Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
Journal (Genetics)
Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
Journal (Genetics)
Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
 
Click here for the English version

使用声学纳米分配技术实现高通量DNA质粒多路复用和转染

Article DOI: 10.3791/59570-v • 13:27 min • August 8th, 2019
August 8th, 2019 •
1, 2, 1, 1
1Institut Pasteur de Lille, Institut National de la Santé et de la Recherche Médicale (Inserm) U1177, Drugs and Molecules for Living Systems, Université de Lille, 2L'atelier du Développeur, Marquette lez Lille

Capítulos

Resumo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

该协议使用声滴喷射技术描述了384孔板中哺乳动物细胞的高通量质粒转染。耗时、易出错的 DNA 点胶和复用,以及转染试剂点胶,由软件驱动,由纳米点胶设备执行。然后,这些细胞被播种在这些预填充的井中。

Tags

回缩,问题150,声波滴喷射,高通量,转染,DNA质粒,哺乳动物细胞,液体处理,荧光蛋白
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter