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Journal / Biochemistry / 使用膜内消化技术评估蛋白质与蛋白质相互作用…
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Biochemistry

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JoVE Journal Biochemistry
使用膜内消化技术评估蛋白质与蛋白质相互作用
Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET
Journal (Biochemistry)
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Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
Journal (Biochemistry)
Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
 
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使用膜内消化技术评估蛋白质与蛋白质相互作用

Article DOI: 10.3791/59733-v • 07:07 min • July 19th, 2019
July 19th, 2019 •
*1, *2, 1, 2, 1
1Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, 2Department of Biochemistry, Showa University School of Medicine
* These authors contributed equally

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在这里,我们提出了一个方案,用于制备质谱样本的膜上消化技术。该技术有助于方便分析蛋白质与蛋白质的相互作用。

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生物化学 问题 149 免疫沉淀 LC/MS/MS 蛋白质组学 蛋白质相互作用 PVDF膜 筛选
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