Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Uso de embriões congelados para produção de ratos …
JoVE Journal
Genetics

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Genetics
Uso de embriões congelados para produção de ratos geneticamente modificados
Collection of F1 Generation Caenorhabditis elegans for Chromatin Immunoprecipitation (ChIP) (Video) | JoVE
Journal (Genetics)
Collection of F1 Generation Caenorhabditis elegans for Chromatin Immunoprecipitation (ChIP) (Video) | JoVE
Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry
Journal (Genetics)
Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry
 
Click here for the English version

Uso de embriões congelados para produção de ratos geneticamente modificados

Article DOI: 10.3791/60808-v • 06:46 min • April 2nd, 2020
April 2nd, 2020 •
*1,2,3, *4,5, 6,7, 8,9,10, 8,11, 3, 8,9,10,12,13, 1
1Max Planck Florida Institute for Neuroscience, 2Life Science Research Center, University of Toyama, 3Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, 4Graduate School of Innovative Life Science, University of Toyama, 5Department of Biochemistry, Faculty of Pharmacy, Cairo University, 6Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 7Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology, Jichi Medical University, 8Synthetic Biology Division, Research Center for Advanced Science and Technology, University of Tokyo, 9Institute for Advanced Biosciences, Keio University, 10Graduate School of Media and Governance, Keio University, 11Department of Molecular Oncology, Graduate School of Medicine, Chiba University, 12Department of Biological Sciences, School of Science, University of Tokyo, 13College of Arts and Sciences, University of Tokyo
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Aqui, apresentamos um método modificado para criopreservação de embriões unicelulares, bem como um protocolo que acoplar o uso de embriões congelados e eletroporação para a geração eficiente de camundongos geneticamente modificados.

Tags

Genética Edição 158 CRISPR/Cas9 criopreservação de embriões de uma célula eletroporação camundongos geneticamente modificados GM edição de genomas FBS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter