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Journal / Genetics / आनुवंशिक रूप से संशोधित चूहों के उच्च दक्षता…
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JoVE Journal Genetics
आनुवंशिक रूप से संशोधित चूहों के उच्च दक्षता उत्पादन के लिए फ्रीज-गल भ्रूण का उपयोग
The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
Journal (Genetics)
The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
Journal (Genetics)
Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
 
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आनुवंशिक रूप से संशोधित चूहों के उच्च दक्षता उत्पादन के लिए फ्रीज-गल भ्रूण का उपयोग

Article DOI: 10.3791/60808-v • 06:46 min • April 2nd, 2020
April 2nd, 2020 •
*1,2,3, *4,5, 6,7, 8,9,10, 8,11, 3, 8,9,10,12,13, 1
1Max Planck Florida Institute for Neuroscience, 2Life Science Research Center, University of Toyama, 3Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, 4Graduate School of Innovative Life Science, University of Toyama, 5Department of Biochemistry, Faculty of Pharmacy, Cairo University, 6Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 7Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology, Jichi Medical University, 8Synthetic Biology Division, Research Center for Advanced Science and Technology, University of Tokyo, 9Institute for Advanced Biosciences, Keio University, 10Graduate School of Media and Governance, Keio University, 11Department of Molecular Oncology, Graduate School of Medicine, Chiba University, 12Department of Biological Sciences, School of Science, University of Tokyo, 13College of Arts and Sciences, University of Tokyo
* These authors contributed equally

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यहां, हम एक कोशिका भ्रूण के क्रायोप्रिजर्वेशन के साथ-साथ एक प्रोटोकॉल के लिए एक संशोधित विधि पेश करते हैं जो आनुवंशिक रूप से संशोधित चूहों की कुशल पीढ़ी के लिए फ्रीज-गल भ्रूण और इलेक्ट्रोपोराशन का उपयोग जोड़ता है।

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आनुवंशिकी अंक १५८ CRISPR/Cas9 एक सेल भ्रूण क्रायोप्रिजर्वेशन इलेक्ट्रोपोराशन आनुवंशिक रूप से संशोधित चूहों जीएम जीनोम संपादन FBS
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