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使用微流体腔系统在运动神经元培养物中有机细胞的斧头迁移
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Neurociência
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JoVE Journal Neurociência
Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System
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使用微流体腔系统在运动神经元培养物中有机细胞的斧头迁移

DOI:
10.3791/60993-v

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10:12 min

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May 05, 2020

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, , , ,
1Department of Physiology and Pharmacology, Sackler Faculty of Medicine,Tel Aviv University, 2Sagol School of Neuroscience,Tel Aviv University

Capítulos

  • 00:04Introduction
  • 01:08Microfluidic Chamber Preparation
  • 05:53Neuronal Culture Plating
  • 07:24Axonal Transport
  • 08:19Results: Axonal Transport of Mitochondria and Acidic Compartments in Motor Neurons
  • 09:34Conclusion

Summary

Tadução automática

Tadução automática

斧头迁移是运动神经元健康的重要机制。在该协议中，我们提供了一种使用微流体室跟踪运动神经元斧头酸性隔间和线粒体的斧头传输的详细方法。

Tags

Axonal TransportMotor NeuronMicrofluidic ChambersNeurodegenerative DiseaseALSAxonal GrowthAxonal DegenerationPDMSChlorotrimethylsilaneVacuum Desiccator

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