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Isolamento e Cultivo de Células-Tronco Mesenquimais de Medula Óssea Mandibular em Ratos
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Isolamento e Cultivo de Células-Tronco Mesenquimais de Medula Óssea Mandibular em Ratos

Article DOI: 10.3791/61532-v • 08:58 min • August 25th, 2020
August 25th, 2020 •
*1, *1, 1, 1, 1, 1, 2, 1
1Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology, 2The 2nd Dental Center, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology
* These authors contributed equally

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Este artigo apresenta um método que combina a adesão total da medula óssea e a classificação da citometria de fluxo para isolar, cultivar, classificar e identificar células-tronco mesenquimais de medula óssea a partir de mandíbulas de ratos.

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Medicina Edição 162 células-tronco mesenquimais de medula óssea mandibular mBMSCs osteogênese condronese adipogênese cultivo celular classificação celular fluorescente
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