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JoVE Journal Developmental Biology
Um Protocolo para Imunohistoquímica e Distribuição In-situ de RNA dentro do Embrião Drosophila Precoce
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Um Protocolo para Imunohistoquímica e Distribuição In-situ de RNA dentro do Embrião Drosophila Precoce

Article DOI: 10.3791/61776-v • 14:34 min • May 6th, 2022
May 6th, 2022 •
*1, *1, *2,3, 1, 1, 1, 1, 1, 1, 1,4, 1, 5, 6, 7, 3, 1
1National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB, Shaanxi Normal University College of Life Sciences, 2Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine, Xi'an Medical University, 3Ohio State University College of Medicine, 4Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 5University of Pennsylvania ULAR, 6University of Maryland School of Medicine, 7Columbia University Medical Center
* These authors contributed equally

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Aqui, descrevemos um protocolo de detecção e localização da proteína de embrião Drosophila e RNA da coleta à pré-incorporação e incorporação, imunostaining e mRNA in situ hibridização.

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Biologia do Desenvolvimento Questão 183 Embrião de Drosophila seção de parafina slide de revestimento incorporação hibridização in-situ DmFKBP12/Calstabin
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