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Immunology and Infection

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JoVE Journal Immunology and Infection
使用下一代测序的 T 和 B 细胞受体免疫再剧目分析
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Journal (Immunology and Infection)
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A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model
Journal (Immunology and Infection)
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使用下一代测序的 T 和 B 细胞受体免疫再剧目分析

Article DOI: 10.3791/61792-v • 08:59 min • January 12th, 2021
January 12th, 2021 •
1,2, 3,4, 1, 3,4, 1,2
1Pediatric Gastroenterology Unit, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, 2Sackler Faculty of Medicine, Tel Aviv University, 3Cancer Research Center, Sheba Medical Center, 4Molecular Hematology Laboratory, Sheba Medical Center

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当前协议描述了从血液样本和肠道活检中分离脱氧核糖核酸的方法、用于下一代测序的 TCR® 和 IGH PCR 库的生成、NGS 运行的性能和基本数据分析。

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本月在 Jove， 第 167 期， 下一代测序， TCR®， IGH， 免疫剧目， 自适应免疫， T 细胞， B 细胞
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