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Analyse af stentors motilitetsmønstre under og efter oral apparatregenerering ved hjælp af cellesporing
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Analyse af stentors motilitetsmønstre under og efter oral apparatregenerering ved hjælp af cellesporing

Article DOI: 10.3791/62352-v • 07:17 min • April 26th, 2021
April 26th, 2021 •
1, 2, 3, 4, 4
1W. M. Keck Science Department, Scripps, Pitzer, and Claremont McKenna of The Claremont Colleges, 2W. M. Keck Science Department, Pitzer College, 3W. M. Keck Science Department, Scripps College, 4Department of Biochemistry and Biophysics, School of Medicine, University of California at San Francisco

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Vi præsenterer en protokol til karakterisering af bevægelighed og opførsel af en population på hundrede mikron- til millimeterstore celler ved hjælp af brightfieldmikroskopi og cellesporing. Dette assay afslører, at Stentor coeruleus overgår gennem fire adfærdsmæssigt forskellige faser, når man regenererer et tabt oralt apparat.

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Biologi udgave 170 Stentor coeruleus motilitet regenerering fænotypescreening mikroskopi timelapse cilia
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