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Een geoptimaliseerd O9-1/Hydrogel-systeem voor het bestuderen van mechanische signalen in neurale crest-cellen

Article DOI: 10.3791/62693-v • 11:02 min • August 13th, 2021
August 13th, 2021 •
*1, *1, 1,2, 3, 3, 1, 1,2
1Department of Pediatrics, McGovern Medical School, The University of Texas Health Science Center at Houston, 2The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, The University of Texas Health Science Center at Houston, 3SEM AFM Core in the Houston Methodist Hospital Research Institute
* These authors contributed equally

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Gedetailleerde stapsgewijze protocollen worden hier beschreven voor het bestuderen van mechanische signalen in vitro met behulp van multipotente O9-1 neurale crest cellen en polyacrylamide hydrogels van verschillende stijfheid.

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Ontwikkelingsbiologie neurale crest cellen mechanische signalen O9-1 cellen atoomkracht microscopie
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