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Immunology and Infection

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JoVE Journal Immunology and Infection
Monitoreo en tiempo real de la respiración mitocondrial en células T primarias humanas diferenciadas por citoquinas
Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)
Journal (Immunology and Infection)
Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)
Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System
Journal (Immunology and Infection)
Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System
 
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Monitoreo en tiempo real de la respiración mitocondrial en células T primarias humanas diferenciadas por citoquinas

Article DOI: 10.3791/62984-v • 06:55 min • October 19th, 2021
October 19th, 2021 •
*1, *1, 1, 1, 1,3, 2, 1
1National Center for Cancer Immune Therapy, Department of Oncology, University Hospital Herlev, 2Department of Cellular and Molecular Medicine, Center for Healthy Aging, University of Copenhagen, 3Department of Immunology and Microbiology, Inflammation and Cancer Group, University of Copenhagen
* These authors contributed equally

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La adaptación metabólica es fundamental para las células T, ya que dicta la diferenciación, la persistencia y la citotoxicidad. Aquí, se presenta un protocolo optimizado para monitorear la respiración mitocondrial en células T primarias humanas diferenciadas por citoquinas ex vivo .

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Este mes en JoVE Número 176
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