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JoVE Journal Cancer Research
Gepooltes shRNA-Bibliotheksscreening zur Identifizierung von Faktoren, die einen Phänotyp der Arzneimittelresistenz modulieren
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Gepooltes shRNA-Bibliotheksscreening zur Identifizierung von Faktoren, die einen Phänotyp der Arzneimittelresistenz modulieren

Article DOI: 10.3791/63383-v • 14:51 min • June 17th, 2022
June 17th, 2022 •
1,2, 1,2, 2,3, 2,3, 1,2,3, 1,2,3
1Department of Clinical Haematology, Christian Medical College, 2Department of Biotechnology, Thiruvalluvar University, 3Centre for Stem Cell Research, Christian Medical College

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Das RNAi-Screening (High-Throughput-RNA-Interferenz) unter Verwendung eines Pools lentiviraler shRNAs kann ein Werkzeug sein, um therapeutisch relevante synthetische tödliche Ziele in Malignomen nachzuweisen. Wir bieten einen gepoolten shRNA-Screening-Ansatz zur Untersuchung der epigenetischen Effektoren bei akuter myeloischer Leukämie (AML).

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Krebsforschung Ausgabe 184 Hochdurchsatz-Screening RNAi-Screening shRNA epigenetischer Faktor Cytarabin AraC-Resistenz
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