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Screening della libreria di shRNA in pool per identificare i fattori che modulano un fenotipo di resistenza ai farmaci

Article DOI: 10.3791/63383-v • 14:51 min • June 17th, 2022
June 17th, 2022 •
1,2, 1,2, 2,3, 2,3, 1,2,3, 1,2,3
1Department of Clinical Haematology, Christian Medical College, 2Department of Biotechnology, Thiruvalluvar University, 3Centre for Stem Cell Research, Christian Medical College

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Lo screening dell'interferenza dell'RNA ad alto rendimento (RNAi) utilizzando un pool di shRNA lentivirali può essere uno strumento per rilevare bersagli letali sintetici terapeuticamente rilevanti nelle neoplasie maligne. Forniamo un approccio di screening dello shRNA aggregato per studiare gli effettori epigenetici nella leucemia mieloide acuta (LMA).

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Cancer Research Screening ad alto rendimento screening RNAi shRNA fattore epigenetico citarabina resistenza AraC
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