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JoVE Journal Developmental Biology
Isolering af helcelleproteinlysater fra musens ansigtsprocesser og dyrkede palatale mesenchymceller til fosfoproteinanalyse
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Isolering af helcelleproteinlysater fra musens ansigtsprocesser og dyrkede palatale mesenchymceller til fosfoproteinanalyse

Article DOI: 10.3791/63834-v • 07:26 min • April 1st, 2022
April 1st, 2022 •
*1, *1, 1
1Department of Craniofacial Biology, School of Dental Medicine, University of Colorado Anschutz Medical Campus
* These authors contributed equally

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Protokollen præsenterer en metode til isolering af helcelleproteinlysater fra dissekerede museembryo-ansigtsprocesser eller dyrkede museembryonale palatale mesenchymceller og udfører efterfølgende western blotting for at vurdere fosforylerede proteinniveauer.

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Udviklingsbiologi udgave 182 Kraniofacial udvikling fosforylering phosphoproteiner mus maksillære processer museembryonale palatale mesenchymeceller
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