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JoVE Journal Bioengineering
Indkøb og decellularisering af rottebagben ved hjælp af en ex vivo perfusionsbaseret bioreaktor til vaskulariseret sammensat allotransplantation
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Indkøb og decellularisering af rottebagben ved hjælp af en ex vivo perfusionsbaseret bioreaktor til vaskulariseret sammensat allotransplantation

Article DOI: 10.3791/64069-v • 08:50 min • June 9th, 2022
June 9th, 2022 •
1,2, 1,3, 1,2,4
1Latner Thoracic Research Surgical Laboratories, University Health Network, Toronto General Hospital, 2Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, 3Institute of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 4Division of Plastic & Reconstructive Surgery, Department of Surgery, University of Toronto

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Vi beskriver den kirurgiske teknik og decellulariseringsproces for sammensatte rottebagben. Decellularisering udføres ved anvendelse af natriumdodecylsulfat med lav koncentration gennem et ex vivo-maskinperfusionssystem .

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