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Immunology and Infection

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JoVE Journal Immunology and Infection
在气液界面上生长的原代鼻上皮细胞的感染，以表征人类冠状病毒与宿主的相互作用
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在气液界面上生长的原代鼻上皮细胞的感染，以表征人类冠状病毒与宿主的相互作用

Article DOI: 10.3791/64868-v • 09:02 min • September 22nd, 2023
September 22nd, 2023 •
1,2, 1,2, 3,4, 1,2, 3,4
1Department of Microbiology, University of Pennsylvania, 2Penn Center for Research on Coronaviruses and Other Emerging Pathogens, Perelman School of Medicine, University of Pennsylvania, 3Otorhinolaryngology-Head and Neck Surgery, University of Pennsylvania, 4Corporal Michael J. Crescenz VA Medical Center

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Click here for the English version.

鼻上皮是所有呼吸道病原体遇到的主要屏障部位。在这里，我们概述了使用作为气液界面 （ALI） 培养物生长的原代鼻上皮细胞来表征生理相关系统中人类冠状病毒与宿主相互作用的方法。

Tags

感染， 原代鼻上皮细胞， 气液界面， 人冠状病毒， 宿主相互作用， SARS-CoV， MERS-CoV， SARS-CoV-2， HCoV-NL63， HCoV-229E， HCoV-OC43， HCoV-HKU1， 呼吸道， 鼻上皮， 患者来源的鼻腔样本， 宿主-病原体相互作用， 气道， 纤毛功能， 粘液产生， 病毒复制， 宿主细胞嗜性， 病毒诱导的细胞毒性， 先天免疫诱导， 抗病毒治疗
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