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Aislamiento y purificación de células endoteliales microvasculares y macrovasculares a partir de muestras derivadas de pulmón

Article DOI: 10.3791/64885-v • 05:26 min • February 3rd, 2023
February 3rd, 2023 •
1,2, 2, 2,3, 2,3, 1, 4, 4,5, 1,2, 2,3, 1, 6,7, 1, 1,2
1Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 2Center on Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, 3Department of Medicine and Aging Sciences, University “G. d’Annunzio” of Chieti-Pescara, 4U.O. di Chirurgia Toracica e dei Trapianti di Polmone, Fondazione IRCCS Ca’ Granda - Ospedale Maggiore Policlinico di Milano, 5Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti, Università degli Studi di Milano, 6Department of Pharmaceutical Sciences, Università degli Studi di Milano, 7Unit of Cell and Molecular Biology in Cardiovascular Diseases, Centro Cardiologico Monzino IRCCS

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Aunque es un desafío, el aislamiento de las células endoteliales pulmonares es esencial para los estudios sobre la inflamación pulmonar. El presente protocolo describe un procedimiento para el aislamiento de alto rendimiento y alta pureza de células endoteliales macrovasculares y microvasculares.

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Este mes en JoVE Número 192 Células endoteliales explante endotelio pulmones aislamiento celular inflamación
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