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Aislamiento de células endoteliales de la luz de arterias carótidas de ratón para experimentos multiómicos unicelulares

Article DOI: 10.3791/63128-v • 09:12 min • October 4th, 2021
October 4th, 2021 •
*1, *1, 1, 1, 1, 1,2
1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 2Division of Cardiology, Georgia Institute of Technology and Emory University
* These authors contributed equally

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Presentamos un método para aislar células endoteliales y núcleos de la luz de arterias carótidas de ratón expuestas a condiciones de flujo estables o alteradas para realizar experimentos ómicos unicelulares.

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Biología Número 176 Células arteriales Células endoteliales ARNseq unicelular ATACseq unicelular Aterosclerosis
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