Name: Desenvolvimento de um modelo pré-clínico de inalação para testar destilados de cannabis vaporizados (Vídeo) | JoVE Journal
Uploaded: 2025-05-30T13:55:00.000+00:00
Duration: 6 min 9 s
Description: Aprenda a desenvolver um modelo de inalação pré-clínico em camundongos para testar destilados de cannabis vaporizados. Este método permite avaliar alterações transcricionais em células epiteliais pulmonares e níveis séricos de THC-COOH.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Spindle, T. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acute+Effects+of+Smoked+and+Vaporized+Cannabis+in+Healthy+Adults+Who+Infrequently+Use+Cannabis%3A+A+Crossover+Trial%5BTitle%5D)+AND+%22JAMA+Netw+Open%22%5BJournal%5D)">Acute Effects of Smoked and Vaporized Cannabis in Healthy Adults Who Infrequently Use Cannabis: A Crossover Trial.</a> JAMA Netw Open. 1 (7), e184841-e184841 (2018).</li>
<li>Morean, M. E., Kong, G., Camenga, D. R., Cavallo, D. A., Krishnan-Sarin, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High+School+Students%27+Use+of+Electronic+Cigarettes+to+Vaporize+Cannabis%5BTitle%5D)+AND+%22Pediatrics%22%5BJournal%5D)">High School Students' Use of Electronic Cigarettes to Vaporize Cannabis.</a> Pediatrics. 136 (4), 611-616 (2015).</li>
<li>Russell, C., Rueda, S., Room, R., Tyndall, M., Fischer, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Routes+of+administration+for+cannabis+use+-+basic+prevalence+and+related+health+outcomes%3A+A+scoping+review+and+synthesis%5BTitle%5D)+AND+%22Int+J+Drug+Policy%22%5BJournal%5D)">Routes of administration for cannabis use - basic prevalence and related health outcomes: A scoping review and synthesis.</a> Int J Drug Policy. 52, 87-96 (2018).</li>
<li>Lazarjani, M. P., Young, O., Kebede, L., Seyfoddin, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Processing+and+extraction+methods+of+medicinal+cannabis%3A+a+narrative+review%5BTitle%5D)+AND+%22J+Cannabis+Res%22%5BJournal%5D)">Processing and extraction methods of medicinal cannabis: a narrative review.</a> J Cannabis Res. 3 (1), 32(2021).</li>
<li>Jones, C. B., Hill, M. L., Pardini, D. A., Meier, M. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Prevalence+and+correlates+of+vaping+cannabis+in+a+sample+of+young+adults%5BTitle%5D)+AND+%22Psychol+Addict+Behav%22%5BJournal%5D)">Prevalence and correlates of vaping cannabis in a sample of young adults.</a> Psychol Addict Behav. 30 (8), 915-921 (2016).</li>
<li>2023–24 Departmental Plan: Health Canada. , 
 <a target="_blank" href="https://www.canada.ca/en/health-canada/corporate/transparency/corporate-management-reporting/report-plans-priorities/2023-2024-departmental-plan.html">https://www.canada.ca/en/health-canada/corporate/transparency/corporate-management-reporting/report-plans-priorities/2023-2024-departmental-plan.html</a> (2023).</li>
<li>Geweda, M. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evaluation+of+dispensaries%27+cannabis+flowers+for+accuracy+of+labeling+of+cannabinoids+content%5BTitle%5D)+AND+%22J+Cannabis+Res%22%5BJournal%5D)">Evaluation of dispensaries' cannabis flowers for accuracy of labeling of cannabinoids content.</a> J Cannabis Res. 6 (1), 11(2024).</li>
<li>Meehan-Atrash, J., Rahman, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cannabis+Vaping%3A+Existing+and+Emerging+Modalities%2C+Chemistry%2C+and+Pulmonary+Toxicology%5BTitle%5D)+AND+%22Chem+Res+Toxicol%22%5BJournal%5D)">Cannabis Vaping: Existing and Emerging Modalities, Chemistry, and Pulmonary Toxicology.</a> Chem Res Toxicol. 34 (10), 2169-2179 (2021).</li>
<li>Van der Kooy, F., Pomahacova, B., Verpoorte, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cannabis+smoke+condensate+I%3A+the+effect+of+different+preparation+methods+on+tetrahydrocannabinol+levels%5BTitle%5D)+AND+%22Inhal+Toxicol%22%5BJournal%5D)">Cannabis smoke condensate I: the effect of different preparation methods on tetrahydrocannabinol levels.</a> Inhal Toxicol. 20 (9), 801-804 (2008).</li>
<li>Rosenberg, E. C., Patra, P. H., Whalley, B. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Therapeutic+effects+of+cannabinoids+in+animal+models+of+seizures%2C+epilepsy%2C+epileptogenesis%2C+and+epilepsy-related+neuroprotection%5BTitle%5D)+AND+%22Epilepsy+Behav%22%5BJournal%5D)">Therapeutic effects of cannabinoids in animal models of seizures, epilepsy, epileptogenesis, and epilepsy-related neuroprotection.</a> Epilepsy Behav. 70 (Pt B), 319-327 (2017).</li>
<li>National Academies of Sciences, Engineering, and Medicine; Health and Medicine Division. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22The+Health+Effects+of+Cannabis+and+Cannabinoids%3A+The+Current+State+of+Evidence+and+Recommendations+for+Research%22">The Health Effects of Cannabis and Cannabinoids: The Current State of Evidence and Recommendations for Research</a>. , (2017).</li>
<li>Been, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chronic+low-level+JUUL+aerosol+exposure+causes+pulmonary+immunologic%2C+transcriptomic%2C+and+proteomic+changes%5BTitle%5D)+AND+%22Faseb+J%22%5BJournal%5D)">Chronic low-level JUUL aerosol exposure causes pulmonary immunologic, transcriptomic, and proteomic changes.</a> Faseb J. 37 (2), e22732(2023).</li>
<li>Been, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Differential+impact+of+JUUL+flavors+on+pulmonary+immune+modulation+and+oxidative+stress+responses+in+male+and+female+mice%5BTitle%5D)+AND+%22Arch+Toxicol%22%5BJournal%5D)">Differential impact of JUUL flavors on pulmonary immune modulation and oxidative stress responses in male and female mice.</a> Arch Toxicol. 96 (6), 1783-1798 (2022).</li>
<li>Caruana, V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chronic+exposure+to+E-cigarette+aerosols+potentiates+atherosclerosis+in+a+sex-dependent+manner%5BTitle%5D)+AND+%22Toxicol+Appl+Pharmacol%22%5BJournal%5D)">Chronic exposure to E-cigarette aerosols potentiates atherosclerosis in a sex-dependent manner.</a> Toxicol Appl Pharmacol. 492, 117095(2024).</li>
<li>Paoli, S., Eidelman, D. H., Mann, K. K., Baglole, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Sex-specific+alterations+in+pulmonary+metabolic%2C+xenobiotic+and+lipid+signalling+pathways+after+e-cigarette+aerosol+exposure+during+adolescence+in+mice%5BTitle%5D)+AND+%22BMJ+Open+Respir+Res%22%5BJournal%5D)">Sex-specific alterations in pulmonary metabolic, xenobiotic and lipid signalling pathways after e-cigarette aerosol exposure during adolescence in mice.</a> BMJ Open Respir Res. 11 (1), e002423(2024).</li>
<li>Lamb, T., Muthumalage, T., Meehan-Atrash, J., Rahman, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nose-Only+Exposure+to+Cherry-+and+Tobacco-Flavored+E-Cigarettes+Induced+Lung+Inflammation+in+Mice+in+a+Sex-Dependent+Manner%5BTitle%5D)+AND+%22Toxics%22%5BJournal%5D)">Nose-Only Exposure to Cherry- and Tobacco-Flavored E-Cigarettes Induced Lung Inflammation in Mice in a Sex-Dependent Manner.</a> Toxics. 10 (8), 471(2022).</li>
<li>Wang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Protein+thiol+oxidation+in+the+rat+lung+following+e-cigarette+exposure%5BTitle%5D)+AND+%22Redox+Biol%22%5BJournal%5D)">Protein thiol oxidation in the rat lung following e-cigarette exposure.</a> Redox Biol. 37, 101758(2020).</li>
<li>Gazarov, E. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pharmacokinetics+of+delta-9-tetrahydrocannabinol+following+acute+cannabis+smoke+exposure+in+mice%3B+effects+of+sex%2C+age%2C+and+strain%5BTitle%5D)+AND+%22Front+Pharmacol%22%5BJournal%5D)">Pharmacokinetics of delta-9-tetrahydrocannabinol following acute cannabis smoke exposure in mice; effects of sex, age, and strain.</a> Front Pharmacol. 14, 1227220(2023).</li>
<li>Haidar, Z., Traboulsi, H., Eidelman, D. H., Baglole, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Differential+inflammatory+profile+in+the+lungs+of+mice+exposed+to+cannabis+smoke+with+varying+THC%3ACBD+ratio%5BTitle%5D)+AND+%22Arch+Toxicol%22%5BJournal%5D)">Differential inflammatory profile in the lungs of mice exposed to cannabis smoke with varying THC:CBD ratio.</a> Arch Toxicol. 97 (7), 1963-1978 (2023).</li>
<li>Behar, R. Z., Hua, M., Talbot, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Puffing+topography+and+nicotine+intake+of+electronic+cigarette+users%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Puffing topography and nicotine intake of electronic cigarette users.</a> PLoS One. 10 (2), e0117222(2015).</li>
<li>Leavens, E. L. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((electronic+cigarette+use+patterns%2C+other+tobacco+product+use%2C+and+reasons+for+use+among+ever+users%3A+Results+from+a+convenience+sample%5BTitle%5D)+AND+%22Addict+Behav%22%5BJournal%5D)">electronic cigarette use patterns, other tobacco product use, and reasons for use among ever users: Results from a convenience sample.</a> Addict Behav. 95, 178-183 (2019).</li>
<li>Huestis, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Human+cannabinoid+pharmacokinetics%5BTitle%5D)+AND+%22Chem+Biodivers%22%5BJournal%5D)">Human cannabinoid pharmacokinetics.</a> Chem Biodivers. 4 (8), 1770-1804 (2007).</li>
<li>Hložek, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pharmacokinetic+and+behavioural+profile+of+THC%2C+CBD%2C+and+THC%2BCBD+combination+after+pulmonary%2C+oral%2C+and+subcutaneous+administration+in+rats+and+confirmation+of+conversion+in+vivo+of+CBD+to+THC%5BTitle%5D)+AND+%22Eur+Neuropsychopharmacol%22%5BJournal%5D)">Pharmacokinetic and behavioural profile of THC, CBD, and THC+CBD combination after pulmonary, oral, and subcutaneous administration in rats and confirmation of conversion in vivo of CBD to THC.</a> Eur Neuropsychopharmacol. 27 (12), 1223-1237 (2017).</li>
<li>Nasrin, S., Watson, C. J. W., Perez-Paramo, Y. X., Lazarus, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cannabinoid+Metabolites+as+Inhibitors+of+Major+Hepatic+CYP450+Enzymes%2C+with+Implications+for+Cannabis-Drug+Interactions%5BTitle%5D)+AND+%22Drug+Metab+Dispos%22%5BJournal%5D)">Cannabinoid Metabolites as Inhibitors of Major Hepatic CYP450 Enzymes, with Implications for Cannabis-Drug Interactions.</a> Drug Metab Dispos. 49 (12), 1070-1080 (2021).</li>
<li>Hartman, R. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Controlled+Cannabis+Vaporizer+Administration%3A+Blood+and+Plasma+Cannabinoids+with+and+without+Alcohol%5BTitle%5D)+AND+%22Clin+Chem%22%5BJournal%5D)">Controlled Cannabis Vaporizer Administration: Blood and Plasma Cannabinoids with and without Alcohol.</a> Clin Chem. 61 (6), 850-869 (2015).</li>
<li>Newmeyer, M. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Free+and+Glucuronide+Whole+Blood+Cannabinoids%27+Pharmacokinetics+after+Controlled+Smoked%2C+Vaporized%2C+and+Oral+Cannabis+Administration+in+Frequent+and+Occasional+Cannabis+Users%3A+Identification+of+Recent+Cannabis+Intake%5BTitle%5D)+AND+%22Clin+Chem%22%5BJournal%5D)">Free and Glucuronide Whole Blood Cannabinoids' Pharmacokinetics after Controlled Smoked, Vaporized, and Oral Cannabis Administration in Frequent and Occasional Cannabis Users: Identification of Recent Cannabis Intake.</a> Clin Chem. 62 (12), 1579-1592 (2016).</li>
<li>Schwope, D. M., Karschner, E. L., Gorelick, D. A., Huestis, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Identification+of+recent+cannabis+use%3A+whole-blood+and+plasma+free+and+glucuronidated+cannabinoid+pharmacokinetics+following+controlled+smoked+cannabis+administration%5BTitle%5D)+AND+%22Clin+Chem%22%5BJournal%5D)">Identification of recent cannabis use: whole-blood and plasma free and glucuronidated cannabinoid pharmacokinetics following controlled smoked cannabis administration.</a> Clin Chem. 57 (10), 1406-1414 (2011).</li>
<li>Sharma, P., Murthy, P., Bharath, M. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chemistry%2C+metabolism%2C+and+toxicology+of+cannabis%3A+clinical+implications%5BTitle%5D)+AND+%22Iran+J+Psychiatry%22%5BJournal%5D)">Chemistry, metabolism, and toxicology of cannabis: clinical implications.</a> Iran J Psychiatry. 7 (4), 149-156 (2012).</li>
<li>Metna-Laurent, M., Mondésir, M., Grel, A., Vallée, M., Piazza, P. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cannabinoid-Induced+Tetrad+in+Mice%5BTitle%5D)+AND+%22Curr+Protoc+Neurosci%22%5BJournal%5D)">Cannabinoid-Induced Tetrad in Mice.</a> Curr Protoc Neurosci. 80, 9.59.1-9.59.10 (2017).</li>
<li>Soo, J. C., Monaghan, K., Lee, T., Kashon, M., Harper, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Air+sampling+filtration+media%3A+Collection+efficiency+for+respirable+size-selective+sampling%5BTitle%5D)+AND+%22Aerosol+Sci+Technol%22%5BJournal%5D)">Air sampling filtration media: Collection efficiency for respirable size-selective sampling.</a> Aerosol Sci Technol. 50 (1), 76-87 (2016).</li>
<li>Tang, Z. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cytotoxicity+and+toxicoproteomic+analyses+of+human+lung+epithelial+cells+exposed+to+extracts+of+atmospheric+particulate+matters+on+PTFE+filters+using+acetone+and+water%5BTitle%5D)+AND+%22Ecotoxicol+Environ+Safety%22%5BJournal%5D)">Cytotoxicity and toxicoproteomic analyses of human lung epithelial cells exposed to extracts of atmospheric particulate matters on PTFE filters using acetone and water.</a> Ecotoxicol Environ Safety. 191, 110223(2020).</li>
<li>Puah, P. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Extractable+impurities+from+fluoropolymer-based+membrane+filters+-+interference+in+high-throughput%2C+untargeted+analysis%5BTitle%5D)+AND+%22RSC+Adv%22%5BJournal%5D)">Extractable impurities from fluoropolymer-based membrane filters - interference in high-throughput, untargeted analysis.</a> RSC Adv. 9 (55), 31918-31927 (2019).</li>
<li>Berrada-Gomez, M. P., Bui, B., Bondarenko, H., Ferret, P. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Particle+size+distribution+in+the+evaluation+of+the+inhalation+toxicity+of+cosmetic+spray+products%5BTitle%5D)+AND+%22Reg+Toxicol+Pharmacol%22%5BJournal%5D)">Particle size distribution in the evaluation of the inhalation toxicity of cosmetic spray products.</a> Reg Toxicol Pharmacol. 139, 105359(2023).</li>
<li>Hammer, T., Gao, H., Pan, Z., Wang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Relationship+between+Aerosols+Exposure+and+Lung+Deposition+Dose%5BTitle%5D)+AND+%22Aerosol+Air+Quality+Res%22%5BJournal%5D)">Relationship between Aerosols Exposure and Lung Deposition Dose.</a> Aerosol Air Quality Res. 20 (5), 1083-1093 (2020).</li>
<li>Asgharian, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Computational+modeling+of+nanoscale+and+microscale+particle+deposition%2C+retention+and+dosimetry+in+the+mouse+respiratory+tract%5BTitle%5D)+AND+%22Inhal+Toxicol%22%5BJournal%5D)">Computational modeling of nanoscale and microscale particle deposition, retention and dosimetry in the mouse respiratory tract.</a> Inhal Toxicol. 26 (14), 829-842 (2014).</li>
<li>Chou, L. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Particle+size+matters%3A+Discrepancies+in+the+health+risks+posed+by+traditional+cigarettes+and+e-cigarettes+in+mice+and+humans%5BTitle%5D)+AND+%22J+Hazardous+Mater+Lett%22%5BJournal%5D)">Particle size matters: Discrepancies in the health risks posed by traditional cigarettes and e-cigarettes in mice and humans.</a> J Hazardous Mater Lett. 4, 100088(2023).</li>
<li>Cheng, Y. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Exposing+Animals+to+Oxidant+Gases%5BTitle%5D)+AND+%22Proc+Am+Thorac+Society%22%5BJournal%5D)">Exposing Animals to Oxidant Gases.</a> Proc Am Thorac Society. 7 (4), 264-268 (2010).</li>
<li>Chen, L. C., Lippmann, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Inhalation+Toxicology+Methods%3A+The+Generation+and+Characterization+of+Exposure+Atmospheres+and+Inhalational+Exposures%5BTitle%5D)+AND+%22Curr+Protoc+Toxicol%22%5BJournal%5D)">Inhalation Toxicology Methods: The Generation and Characterization of Exposure Atmospheres and Inhalational Exposures.</a> Curr Protoc Toxicol. 63 (1), 24.24.21-24.24.23 (2015).</li>
<li>Wong, B. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Inhalation+Exposure+Systems%3A+Design%2C+Methods+and+Operation%5BTitle%5D)+AND+%22Toxicologic+Pathol%22%5BJournal%5D)">Inhalation Exposure Systems: Design, Methods and Operation.</a> Toxicologic Pathol. 35 (1), 3-14 (2007).</li>
<li>Cousijn, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Grey+matter+alterations+associated+with+cannabis+use%3A+results+of+a+VBM+study+in+heavy+cannabis+users+and+healthy+controls%5BTitle%5D)+AND+%22Neuroimage%22%5BJournal%5D)">Grey matter alterations associated with cannabis use: results of a VBM study in heavy cannabis users and healthy controls.</a> Neuroimage. 59 (4), 3845-3851 (2012).</li>
<li>Filbey, F. M., Schacht, J. P., Myers, U. S., Chavez, R. S., Hutchison, K. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Marijuana+craving+in+the+brain%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+U+S+A%22%5BJournal%5D)">Marijuana craving in the brain.</a> Proc Natl Acad Sci U S A. 106 (31), 13016-13021 (2009).</li>
<li>Sneider, J. T., Gruber, S. A., Rogowska, J., Silveri, M. M., Yurgelun-Todd, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+preliminary+study+of+functional+brain+activation+among+marijuana+users+during+performance+of+a+virtual+water+maze+task%5BTitle%5D)+AND+%22J+Addict%22%5BJournal%5D)">A preliminary study of functional brain activation among marijuana users during performance of a virtual water maze task.</a> J Addict. 2013, 461029(2013).</li>
<li>Gilman, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cannabis+use+is+quantitatively+associated+with+nucleus+accumbens+and+amygdala+abnormalities+in+young+adult+recreational+users%5BTitle%5D)+AND+%22J+Neurosci%22%5BJournal%5D)">Cannabis use is quantitatively associated with nucleus accumbens and amygdala abnormalities in young adult recreational users.</a> J Neurosci. 34 (16), 5529-5538 (2014).</li>
</ol>

<table><tbody><tr><td>2,2,2-Tribromoetanol</td><td>Sigma-Aldrich</td><td>T48402-5G</td><td>Avertin</td></tr><tr><td>inExpose</td><td>SCIREQ</td><td>sales@scireq.com</td><td>www.scireq.com</td></tr><tr><td>Tubos Separadores de Soro Microtainer</td><td>BD</td><td>365967</td><td></td></tr><tr><td>Pax Era Vape Pen</td><td>PAX</td><td></td><td>Comprado na Loja de Cannabis de Ontário</td></tr><tr><td>Pineapple Express Pax Pod</td><td>Bom Fornecimento</td><td></td><td>Comprado na Loja de Cannabis de Ontário</td></tr><tr><td>SoftRestraints</td><td>SCIREQ</td><td>IX-XN1-SR-AL</td><td>www.scireq.com</td></tr><tr><td>Kit ELISA Forense THC</td><td>Neogen</td><td>131019</td><td></td></tr></tbody></table>

desenvolvimento de um modelo pré-clínico de inalação para testar destilados de cannabis vaporizados

Development of a Preclinical Inhalation Model to Test Vaporized Cannabis Distillates

O objetivo inicial era determinar um regime de exposição que pudesse fornecer níveis fisiologicamente relevantes de THC ao sangue em comparação com os humanos. Assim, um componente-chave para selecionar os parâmetros de exposição foi usar características que imitassem os padrões de uso humano20,21. Camundongos C57BL/6 machos e fêmeas foram expostos a um Pineapple Express Pax Era Pod contendo ~85% de THC por 10 min, 20 min e 30 min...

Desenvolvimento de um modelo pré-clínico de inalação para testar destilados de cannabis vaporizados

Despite their growing popularity, cannabis vape products remain understudied. Cannabis vape cartridges are used with battery-powered devices that ...

development-preclinical-inhalation-model-to-test-vaporized-cannabis

Research

JoVE Journal

Medicine

Medicina

Este protocolo descreve um método para expor roedores ao vapor de cigarro eletrônico (vapor E) e fumaça de cigarro. As câmaras de exposição são construídas modificando câmaras de anestesia com um sistema de bombeamento automatizado que fornece vapor E ou fumaça de cigarro a roedores. Este sistema pode ser facilmente modificado para acomodar muitos endpoints experimentais.

Comparando os efeitos do fumo eletrônico do cigarro e do cigarro em uma novela<em&gt; In Vivo</em&gt; Sistema de Exposição

Aqui, descrevemos um aparelho de laboratório programáveis que pode ser usado para criar extratos de aerossol de cigarro eletrônico e fumaça de cigarro convencional. Este método fornece uma ferramenta útil para fazer comparações diretas entre cigarros convencionais e cigarros eletrônicos e é um ponto de entrada acessível para pesquisa de cigarro eletrônico.

Um microcontrolador operado dispositivo para a geração de extratos líquidos de aerossol de cigarro eletrônico e fumo cigarro convencional

Os usuários de cigarro eletrônico (e-cig) estão a aumentar em todo o mundo. Pouco, no entanto, é conhecido sobre os efeitos de saúde induzidos por aerossóis inalados e-cig. Este artigo descreve uma técnica de geração de aerossol e-cig apropriada para exposições de animais e estudos toxicológicos posteriores. Esses protocolos são necessários para estabelecer sistemas de exposição experimentalmente reproduzíveis e padronizada e-cig.

Geração de aerossol de cigarro eletrônico por um dispositivo de máquina-Vaping de terceira geração: aplicativo para estudos toxicológicos

Apresentamos um sistema robusto, transferível e preditivo de exposição in vitro para a triagem e monitoramento de partículas transmitidas pelo ar sobre sua citotoxicidade pulmonar aguda, expondo células pulmonares humanas cultivadas na interface ar-líquido (ALI).

Avaliação da toxicidade da inalação aguda de partículas transmitidas por expor células pulmonares humanas cultivadas na Interface Ar-Líquido

O protocolo descreve as configurações e o uso de um gerador de aerossol capilar para produção contínua de aerossol controlado a partir de uma solução líquida multiespécie, adequada para entrega constante de aerossol de grande volume (por exemplo, estudos de inalação de inalação viva ).

Uso de Gerador de Aerossol Capilar em Produção Contínua de Aerossol Controlado para Estudos Não Clínicos

O presente protocolo fornece informações instrucionais para o uso do tromfo de tabaco Manduca sexta na pesquisa canabinoide.  O método descrito aqui inclui todos os suprimentos e protocolos necessários para monitorar alterações fisiológicas e comportamentais do modelo de insetos em resposta ao tratamento do canabidiol (CBD).

Minhoca de Tabaco como um sistema modelo de insetos para estudos pré-clínicos canabinoides

Estabelecemos um modelo in vitro padronizado para estudar a exposição ao vapor de cannabis na interface ar-líquido. Este modelo fornece uma abordagem sistemática para examinar os impactos da cannabis vaporizada, abordando o crescente interesse em vaporizadores de cannabis como uma alternativa ao fumo.

In vitro Exposições à Cannabis de Células Epiteliais Pulmonares na Interface Ar-Líquido

A administração oral via gavagem em roedores pode induzir sofrimento associado à contenção. Exploramos a administração oromucosa/bucal de extrato de Cannabis enriquecido com CBD, onde os ratos foram tratados por 15 dias com o extrato a 3 mg/kg/dia. Os tratamentos foram administrados por via oral com micropipeta, utilizando-se a técnica de toque afetuoso para manipular os animais.

Oromucosa como método alternativo para administração de produtos de cannabis em roedores

À medida que as restrições legislativas mudam, resultando em maior acessibilidade aos produtos canabinóides, é extremamente importante desenvolver modelos para estudar o impacto dessas exposições, principalmente durante a gravidez. Um modelo pré-clínico de exposição pré-natal moderada a canabinóides por meio de ingestão voluntária foi desenvolvido, permitindo investigações mais aprofundadas.

Modelo pré-clínico de exposição pré-natal ao delta-9-tetrahidrocanabinol para avaliar seu impacto nos resultados do neurodesenvolvimento

Δ 9-tetrahidrocanabinol (THC) é o constituinte intoxicante da cannabis e uma substância controlada nos Estados Unidos. Este artigo descreve os procedimentos padrão para obter, armazenar e administrar THC a camundongos em conformidade com os regulamentos legais atuais e apresenta brevemente um método para avaliar os efeitos farmacodinâmicos básicos do THC em roedores.

Administração de Δ 9-tetrahidrocanabinol (THC) em camundongos adolescentes e adultos

Aprenda a desenvolver um modelo de inalação pré-clínico em camundongos para testar destilados de cannabis vaporizados. Este método permite avaliar alterações transcricionais em células epiteliais pulmonares e níveis séricos de THC-COOH.

Desenvolvimento de um modelo pré-clínico de inalação para testar destilados de cannabis vaporizados

Explorar mais vídeos