Flow Cell Priming
Flow Cell Priming - Related Videos
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Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
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This technique provides a method to harvest, normalize and quantify intracellular growth of bacterial pathogens that are pre-cultivated in natural protozoan host cells prior to infections of mammalian cells. This method can be modified to accommodate a wide variety of host cells for the priming stage as well as target cell types.
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Verbal Priming
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Source: Laboratory of Jonathan Flombaum—Johns Hopkins University Human memory seems to work in two broad ways. Like modern computers, the human mind has explicit, or declarative, memory: ask a question, and a person gives the best answer they can. Input a query, and a computer program returns the contents of the relevant parts of its stored memory. Humans also have a second kind of memory system, one not really typical of computers, one that experimental psychologists call implicit. Implicit...
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FAQs
Replacing the storage solution with the priming solution establishes the chemical environment needed for the assay or sequencing run. This exchange also helps prepare the flow cell for sample introduction rather than leaving it in its storage condition. When the replacement is incomplete, the resulting environment may be less consistent across active channels and measurements can become more variable.
Trapped air can interrupt fluid contact with parts of the flow cell, including active channels and the membrane. Introducing priming solution helps displace these air pockets and promotes more continuous fluidic access. This matters because incomplete contact can reduce channel availability, making sample loading less consistent and potentially weakening the reliability of signals generated during the experiment.
Membrane equilibration allows the membrane to reach conditions suitable for the planned measurement, while establishing appropriate ionic conditions supports signal generation across active channels. These two effects are linked: a membrane that has not equilibrated, or channels exposed to unsuitable ionic conditions, may produce less consistent analytical behavior. Priming therefore prepares both the physical membrane environment and its chemical surroundings.
A basic workflow introduces the designated priming solution through the flow cell before the assay or sequencing run. The solution replaces the storage solution, displaces trapped air, equilibrates the membrane, and establishes the intended ionic conditions. After these preparatory changes occur, the flow cell is ready for more consistent sample loading and measurement.
Priming is especially important before biochemical assays or nanopore sequencing runs that depend on membrane-based analytical platforms. In these settings, the flow cell must present a suitable fluidic and chemical environment before samples are loaded. Careful preparation improves reproducibility between runs and helps researchers distinguish experimental signals from variability caused by incomplete preparation.
Incomplete priming can leave storage solution, trapped air, or unsuitable local conditions within the flow cell. These problems may reduce the number of available channels and make sample loading less uniform. The resulting measurements can show greater variability, so poor data quality may reflect inadequate preparation rather than differences in the biochemical sample itself.