Electric Pulse Stimulation
Electric Pulse Stimulation - Related Videos
07:29
Programmed Electrical Stimulation in Mice
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Cited by 48 •
2010
Programmed electrical stimulation provides the ability to determine conduction properties of the heart, and the possibility to induce and terminate cardiac arrhythmias using various pacing protocols. Using a transvenous catheter, intracardiac electrogram recordings can be obtained in mice following programmed electrical stimulation protocols to identify arrhythmogenic substrates.
04:46
Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro
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Cited by 2 •
2011
Gene transfection by electroporation is improved approximately two times when orientation of electric field is changed during pulse application, while cell viability is not affected. The increase in gene transfection is caused by the increase of the membrane area which is made competent for DNA entry into the cell.
02:15
Regulating Schwann Cell Growth In Vitro Using the Nanosecond Pulsed Electric Field Technique
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2025
Source: Han, J., et.al. Regulating Schwann Cell Growth by Nanosecond Pulsed Electric Field for Peripheral Nerve Regeneration In Vitro. J. Vis. Exp. (2024)This video demonstrates the application of the nanosecond pulsed electric field (nsPEF) technique to modulate Schwann cell (SC) growth. The rapid electric pulses induce transient nanopores in SC membranes, facilitating calcium influx that activates signaling pathways driving SC proliferation, dedifferentiation, and neurotrophic factor...
07:53
Assessment of Neuromuscular Function Using Percutaneous Electrical Nerve Stimulation
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Cited by 30 •
2015
We present a protocol to assess changes in neuromuscular function. Percutaneous electrical nerve stimulation is a non-invasive method that evokes muscular responses. Electrophysiological and mechanical properties of these responses permit the evaluation of neuromuscular function from brain to muscle (supra-spinal, spinal and peripheral levels).
08:24
A Murine Model of Muscle Training by Neuromuscular Electrical Stimulation
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Cited by 18 •
2012
A murine model of neuromuscular electrical stimulation (NMES), a safe and inexpensive clinical modality, to the anterior compartment muscles is described. This model has the advantage of modifying a readily available clinical device for the purpose of eliciting targeted and specific muscle contractions in mice.
FAQs
The main controllable variables are pulse amplitude, duration, frequency, and waveform. Together, they determine how strongly and how often transmembrane voltage changes occur, which helps explain why identical biological samples can produce different responses under different stimulation settings. Precise control of these parameters is therefore central to reproducible experiments and safe applications.
Electrical pulses can alter transmembrane voltage, the voltage difference across a cell membrane. This change may activate ion channels, which then participate in intracellular signaling. The resulting cellular response depends on the applied signal rather than on electrical input alone, linking pulse design to outcomes such as muscle contraction or changes in growth and differentiation.
Changing pulse amplitude, duration, frequency, or waveform can shift the biological response because these settings control the electrical conditions experienced by the sample. Considering the full parameter set helps researchers interpret differences between experiments and optimize stimulation. This is especially important when the goal is to obtain a consistent response from cultured cells, tissues, or engineered constructs.
A practical workflow begins by selecting the biological target, such as cells, tissue, or an engineered construct, and defining the intended response. Researchers then apply controlled pulses while setting amplitude, duration, frequency, and waveform. They can compare resulting contraction, signaling-related behavior, growth, or differentiation across conditions to evaluate how electrical control affects the system.
The technique supports research on excitable tissues and the regulation of cultured muscle and nerve cells. It can also be applied to tissue-engineering studies in which researchers examine how electrical cues influence engineered constructs. These uses make controlled stimulation valuable for investigating cellular behavior and for developing systems that reproduce or guide tissue responses.
Controlled electrical stimulation can inform therapeutic systems designed for rehabilitation and regenerative medicine. Its relevance comes from the ability to influence responses such as muscle contraction, cell growth, and differentiation while adjusting the pulse conditions. In bioengineering, this provides a way to connect electrical control with tissue-related outcomes and to study how engineered systems may support recovery or regeneration.