Eukaryotic Microorganisms

Eukaryotic microorganisms are microscopic organisms whose cells contain a nucleus and membrane-bound organelles, distinguishing them from prokaryotes and making them important models for understanding cellular organization. Their compartmentalized cells support specialized processes, including energy production in mitochondria, photosynthesis in chloroplasts in some groups, and regulated growth through mitosis or other reproductive cycles. This diverse category includes yeasts, microscopic algae, protozoa, and some fungi, which occupy essential roles in ecosystems as producers, decomposers, parasites, and symbiotic partners. In biology, they support research on cell biology, evolution, infectious disease, biotechnology, and environmental processes.

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JoVE Core - Molecular Biology

The Eukaryotic Promoter Region

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2020

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...

Eukaryotic RNA Polymerases

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2025

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action. All three eukaryotic RNAPs require specific transcription factors, of which the...

Quantifying Environmental Microorganisms and Viruses Using qPCR

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2023

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Bradley Schmitz Quantitative polymerase chain reaction (qPCR), also known as real-time PCR, is a widely-used molecular technique for enumerating microorganisms in the environment. Prior to this approach, quantifying microorganisms was limited largely to classical culture-based techniques. However, the culturing of microbes from environmental samples can be particularly challenging, and...

The Tree of Life - Bacteria, Archaea, Eukaryotes

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2020

The “tree of life” describes the evolution of life and the evolutionary relationships between organisms. The root of the tree is the common ancestor to all life on Earth. All other species radiate from this point, much like the branches of a tree. The numerous tips of these branches on the tree of life represent every living, or extant, species. Extinct species, which are species that no longer exist, can be found towards the center of the tree. Currently, these organisms, both extant and...

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JoVE Core - Microbiology
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Types of Microorganisms

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2025

Microorganisms are a diverse group of microscopic entities broadly categorized into cellular and acellular types based on their structural organization. Cellular microorganisms include bacteria, archaea, fungi, protozoa, and algae, while acellular microorganisms are represented by viruses.Cellular MicroorganismsBacteriaBacteria, tiny prokaryotic organisms, exhibit fascinating shapes such as rods, spheres, and spirals. They adapt to diverse habitats, including soil, water, and human-associated...

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