Live Dead Enrichment
Live Dead Enrichment - Related Videos
09:58
An Optimized LIVE/DEAD Assay Coupled with Flow Cytometry for Quantifying Post-Stress Survival in Yeast Cells
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Cited by 1 •
2025
Here, we present a protocol to quantify post-stress survival in yeast samples. The assay employs two fluorescent dyes, SYTO 9 and propidium iodide (PI), to quantify plasma membrane integrity. It uses flow cytometry to provide quantitative and reproducible estimates of the live, dead, and damaged cell fractions for post-oxidative-stress samples.
06:29
Live/Dead Staining for Quantifying Viable but Not Culturable Cells in Manuka Honey-Treated Wound-Causing Bacteria
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2025
The presented protocol describes a procedure to quantify Viable But Not Culturable Cells (VBNC) in Manuka Honey-treated bacterial cultures.
01:34
Environmental Enrichment for Rodents
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Environmental enrichment involves objects and practices that encourage natural behaviors, improve psychological well-being, and promote better welfare in laboratory rodents. Environmental enrichment should be tailored to the...
08:04
Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
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2019
A simple ATP-measuring assay and live/dead staining method were used to quantify and visualize Neisseria gonorrhoeae survival after treatment with ceftriaxone. This protocol can be extended to examine the antimicrobial effects of any antibiotic and can be used to define the minimal inhibitory concentration of antibiotics in bacterial biofilms.
10:47
Quantification of Proliferative and Dead Cells in Enteroids
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Cited by 2 •
2020
The presented protocol uses flow cytometry to quantify the number of proliferating and dead cells in cultured mouse enteroids. This method is helpful to evaluate the effects of drug treatment on organoid proliferation and survival.
FAQs
Selection depends on membrane integrity rather than a cancer-specific marker. Intact cells exclude or avoid binding the reagents used for depletion, whereas dead or damaged cells take up viability markers or bind removal reagents. This physical distinction allows the unwanted fraction to be reduced before analysis, making the retained population more suitable for studying cellular characteristics.
Magnetic separation and cell sorting implement the same enrichment principle through different processing formats. Magnetic workflows use removal reagents to support isolation of the unwanted fraction, while cell sorting separates cells according to the relevant viability signal. The appropriate format depends on how the sample will be processed and which downstream analysis requires the enriched live-cell population.
Dead cells and cellular debris can contribute nonviable-cell signals that complicate interpretation. Depleting this material increases the relative quality of the intact-cell fraction available for analysis, which can make measurements more reliable. This benefit is especially relevant when researchers need to characterize mixed tumor or immune populations rather than signals produced by damaged material.
Treatment-response samples may contain both intact cells and cells that have become damaged or nonviable. Reducing the latter helps investigators examine the remaining live-cell material with less interference from nonviable signals. As a result, characterization of tumor cells or immune populations can more clearly reflect the properties of cells retained after treatment-related changes.
The workflow begins with a mixed cell sample containing viable, dead, and damaged material. Viability markers or removal reagents are then applied so the unwanted cells become identifiable or bind the depletion reagents. Magnetic separation or cell sorting is used to separate fractions, and the retained live-cell population proceeds to the selected downstream analysis.
The key components are viability markers or reagents that bind dead or damaged cells, together with a mechanism for separating the resulting fractions. Supported formats include magnetic separation and cell sorting. These components work together to reduce nonviable material while preserving a more useful intact-cell fraction for flow cytometry, molecular profiling, culture, or functional assays.
Enriching live cells can improve several downstream workflows, including flow cytometry, molecular profiling, cell culture, and functional assays. In each case, reducing debris and nonviable-cell signals provides cleaner material for analysis or experimentation. The method is therefore useful when the quality of the starting cell population influences characterization, profiling, growth, or functional readouts.
Cancer research often examines mixed samples containing tumor cells, immune populations, and damaged material. Enriching the intact fraction helps investigators characterize these populations with fewer signals from dead cells and debris. This supports more reliable analysis of cellular features and treatment responses, while providing cleaner material for downstream molecular, culture-based, or functional investigations.