Organic Dye Encapsulation

Organic dye encapsulation is the incorporation of dye molecules within a protective host material, such as a polymer, silica shell, or molecular carrier, to improve their stability and control their behavior. Encapsulation isolates the dye from environmental factors including oxygen, light, solvents, and reactive chemicals through physical confinement or chemical interactions between the dye and host matrix. This approach can reduce photobleaching, limit aggregation, and regulate dye release or accessibility. In chemistry, organic dye encapsulation supports the design of fluorescent probes, sensors, imaging agents, optical materials, and functional coatings with improved performance and longer operational lifetimes.

Organic Dye Encapsulation - Related Videos

Research

JoVE Journal - Chemistry

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

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2015

Organic dye molecules and oleic acid coated upconverting nanoparticles are not water-soluble. This protocol describes a ‘plug and play’ method that enables the transfer of organic dye molecules and upconverting particles from their initial hydrophobic solvent to water.

Research

JoVE Journal - Biology
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Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

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Cited by 54 •

2009

We present protocols for the 3-dimensional (3D) encapsulation of cells within synthetic hydrogels. The encapsulation procedure is outlined for two commonly used methods of crosslinking (michael-type addition and light-initiated free radical mechanisms), as well as a number of techniques for assessing encapsulated cell behavior.

Research

JoVE Journal - Biology
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PuraMatrix Encapsulation of Cancer Cells

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Cited by 31 •

2009

This video demonstrates how to encapsulate and culture cancer cells in PuraMatrix, a commercially available self assembling peptide gel.

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots

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Cited by 6 •

2015

Here, we present a protocol for encapsulation of catabolic cells, which consume lipids for heat production in intra-abdominal adipose tissue and increase energy dissipation in obese mice.

Tracking Hypoxic Signaling within Encapsulated Cell Aggregates

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Cited by 10 •

2011

A method for photo-encapsulation of cells in a crosslinked PEG hydrogel is described. Hypoxic signaling within encapsulated murine insulinoma (MIN6) aggregates is tracked using a fluorescent marker system. This system allows serial examination of cells within a hydrogel scaffold and correlation of hypoxic signaling with changes in cell phenotype.

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