Fluidic Device Culture
Fluidic Device Culture - Related Videos
02:49
Bacterial Culture Filtrate Protein Concentration using an Ultrafiltration Device
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2025
This video demonstrates the isolation and concentration of bacterial culture filtrate proteins, which include bacterial extracellular vesicles, using ultrafiltration. The concentrated extracellular vesicles serve as diagnostic markers for disease detection.
12:32
Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales
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Cited by 2 •
2020
Breakthrough curves (BTCs) are efficient tools to study the transport of bacteria in porous media. Here we introduce tools based on fluidic devices in combination with microscopy and flow cytometric counting to obtain BTCs.
08:01
Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices
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Cited by 10 •
2015
Co-cultures represent a valuable method to study the interactions between nerves and target tissues and organs. Microfluidic systems allow co-culturing ganglia and whole developing organs or tissues in different culture media, thus representing a valuable tool for the in vitro study of the crosstalk between neurons and their targets.
13:36
Scalable Fluidic Injector Arrays for Viral Targeting of Intact 3-D Brain Circuits
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Cited by 10 •
2010
Controlling and analyzing neural circuits in vivo would be facilitated by a technology for delivery of viruses and other reagents to desired 3-dimensional sets of brain regions. We demonstrate customized fluidic injector array fabrication, and delivery of virally-encoded optical sensitizers, enabling optical manipulation of complex brain circuits.
08:29
Thermal Measurement Techniques in Analytical Microfluidic Devices
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Cited by 6 •
2015
Here, we present three protocols for thermal measurements in microfluidic devices.
FAQs
These components determine how liquid enters, moves through, and exits the culture region. Pumps establish flow, valves regulate or program delivery, and channel geometry influences fluid distribution and local exposure. When included, semipermeable barriers can separate compartments while allowing selected exchanges. Together, these features let investigators adjust nutrient delivery, waste removal, oxygen exposure, chemical gradients, and mechanical forces.
Spatial gradients and flow-related forces provide controlled environmental cues that can influence developmental behavior. By changing perfusion patterns, channel arrangements, or barrier-separated compartments, investigators can examine how cells respond to differences in nutrients, oxygen, signaling conditions, or physical stimulation. This helps connect microenvironmental conditions with migration, differentiation, communication, and tissue organization.
Continuous perfusion maintains ongoing liquid exchange, supporting sustained delivery and removal throughout the experiment. Programmed perfusion adds temporal control by changing when or how conditions are delivered. This distinction allows developmental studies to examine responses to stable versus changing environments, while pumps and valves provide the control needed to apply those conditions reproducibly within the device.
A typical setup places cells or tissues in defined device channels, connects the fluidic components, and establishes the intended flow pattern. Researchers then use pumps and valves to provide continuous or programmed perfusion, with channel geometry and semipermeable barriers arranged for the desired exchanges or compartments. The resulting culture can be used to study developmental behaviors under controlled conditions.
These systems support studies of embryonic development, organoid maturation, and disease-related developmental processes. Within the device, researchers can examine how cells communicate, migrate, differentiate, and organize as environmental conditions are regulated. The approach is especially useful when developmental behavior depends on interactions among chemical exposure, oxygen availability, nutrient supply, waste removal, and mechanical conditions.
Compared with static culture, fluidic systems offer tighter control over the cellular microenvironment through regulated exchange and flow. They can improve experimental control, reproducibility, and physiological relevance while using small sample volumes. In developmental biology, these advantages help investigators model tissue formation and organoid maturation under conditions that more closely represent dynamically changing environments than an unchanging culture setting.