Journal
/
Environment
/
Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales
JoVE Journal
Environment
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

DOI:
10.3791/60701-v

•

12:32 min

•

November 25, 2020

•

, , ,
1Stream Biofilm and Ecosystem Research Laboratory,École Polytechnique Fédérale de Lausanne, 2Institute of Earth Sciences,University of Lausanne, CH-1015

Chapters

  • 00:04Introduction
  • 00:57Preparation of A Microfluidic Device in Polydimethylsiloxane (PDMS)
  • 03:27Preparation of a Fluidic Device in Poly(methyl methacrylate)(PMMA)
  • 04:09Analyze Bacterial Transport using PDMS Microfluidic Devices
  • 05:10Basic Image Processing
  • 06:59Analyze Bacterial Transport at the Pore Scale
  • 08:08Study Bacterial Filtration by Means of Deposition Profiles
  • 08:51Analyze Bacterial Transport using PMMA Fluidic Devices
  • 10:33Results: Motile and Nonmotile GFP-tagged P. putida KT2440
  • 11:48Conclusion

Summary

Automatic Translation

Automatic Translation

Breakthrough curves (BTCs) are efficient tools to study the transport of bacteria in porous media. Here we introduce tools based on fluidic devices in combination with microscopy and flow cytometric counting to obtain BTCs.

Tags

Keywords: Microfluidic DevicesMicroscopyFlow CytometryBacterial TransportPorous MediaSpatial ScalesBioremediationContaminationDisease SpreadMathematical ModelsSingle CellPopulationMicrobial CommunityPDMSSU-8 PhotolithographyPorous GeometryFluidic DevicesAutomated DispenserLaboratory-scale Column StudiesField Surveys

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60701?article_type=v

Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity
check_url/60701?article_type=v

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
check_url/60701?article_type=v

Implementation of Portable Emissions Measurement Systems (PEMS) for the Real-driving Emissions (RDE) Regulation in Europe
check_url/60701?article_type=v

Using Pharmacological Manipulation and High-precision Radio Telemetry to Study the Spatial Cognition in Free-ranging Animals
check_url/60701?article_type=v

Dispersion of Nanomaterials in Aqueous Media: Towards Protocol Optimization
check_url/60701?article_type=v

Microfluidic Devices for Characterizing Pore-scale Event Processes in Porous Media for Oil Recovery Applications
check_url/60701?article_type=v

Characterization of Aquatic Biofilms with Flow Cytometry
check_url/60701?article_type=v

Metal Corrosion and the Efficiency of Corrosion Inhibitors in Less Conductive Media
check_url/60701?article_type=v

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
check_url/60701?article_type=v

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Read Article