Fluorescent Cell Imaging
Fluorescent Cell Imaging - Related Videos
07:42
Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging
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Cited by 33 •
2008
This video shows techniques for labeling of human embryonic stem cells and mesenchymal stem cells with fluorescent dyes. This technique can be used for an in vivo tracking of transplanted stem cells with optical imaging and for histopathological correlations with fluorescence microscopy.
19:16
Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
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Cited by 8 •
2009
Selection, microinjection, and imaging of fluorescently-labeled F-actin via fluorescent speckle microscopy (FSM).
06:42
Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
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Cited by 32 •
2013
We review our recent results on the integration of fluorescent microscopy and imaging flow cytometry tools on a cell-phone using compact and cost-effective opto-fluidic attachments. These cell-phone based micro-analysis devices might be useful for cytometric analysis, such as performing various cell counting tasks as well as for high-throughput screening of e.g., water samples in resource limited settings.
09:45
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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Cited by 17 •
2012
Fluorescence Lifetime Imaging (FLIM) has emerged as a key technique to image the environment and interaction of specific proteins and dyes in living cells. FLIM of fluorescent molecular rotors allows mapping of viscosity in living cells.
06:46
Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
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Cited by 4 •
2017
This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation.
FAQs
Fluorescent labels emit light after exposure to excitation at a specific wavelength. The emitted signal provides the detectable information, while a specialized microscope captures it to create an image. This separation between the incoming excitation and resulting emission allows researchers to visualize where labeled cells or cellular components are located within a specimen.
The images provide spatial information about the positions and organization of cells or cellular components, as well as temporal information about changes over time. Examining these dimensions together helps researchers follow dynamic events rather than viewing development as a sequence of isolated structures, supporting analysis of movement, division, and changing tissue organization.
In living specimens, imaging can follow developmental changes as they occur, including cell division, migration, and differentiation. Fixed specimens provide observations of a preserved developmental state instead. Comparing images from living or fixed material allows researchers to examine either ongoing behavior or structural and organizational features at particular developmental stages.
Fluorescent signals can help connect cellular locations and behaviors with developmental outcomes such as gene expression and cell fate. By examining where signals appear and how labeled cells change across developmental stages, researchers can relate cellular activity to differentiation and the eventual organization of tissues within a developing organism.
A general workflow includes preparing a living or fixed specimen with fluorescent labels, exposing the labels to excitation at an appropriate wavelength, and capturing the emitted light with a specialized microscope. Researchers then examine the resulting images for spatial patterns or changes over time, depending on whether the experiment follows one state or multiple developmental stages.
This approach is useful when researchers need to observe how cells divide, migrate, differentiate, or become organized within tissues. It is especially relevant when developmental questions depend on location or timing, because the method supplies images that connect cellular behavior with changes occurring across developmental stages.
Fluorescent cell imaging can support interpretation of tissue organization, morphogenesis, gene expression, and cell fate. Tracking labeled cells and components across stages helps reveal how local cellular changes contribute to larger structural patterns. These observations provide developmental context for understanding the mechanisms that shape developing organisms.