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Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

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Biology

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JoVE Journal Biology

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

DOI:

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19:16 min

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August 05, 2009

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James Lim, Gaudenz Danuser

¹Department of Cell Biology,Scripps Institute

Chapters

00:05Title
00:58Introduction
01:24Preparing Fluorescently Labeled Actin and Cells for Microinjection
05:46Microinjection of Labeled Actin
10:14Assembling the Imaging Chamber
15:20Imaging Cells
18:17Representative Results: Microinjected Cells
18:44Conclusion

Summary

Automatic Translation

Selection, microinjection, and imaging of fluorescently-labeled F-actin via fluorescent speckle microscopy (FSM).

Tags

Fluorescent Speckle Microscopy F-actin Dynamics Live Cell Imaging FSM Actin Dynamics PtK1 Cells Turnover Kinetics Assembly/disassembly Computer Vision Software QFSM Fluorescent Actin Probes Macromolecular Assemblies Microtubules Intermediate Filaments Adhesion Complexes

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