Lps Odn1826
Lps Odn1826 - Related Videos
08:51
Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic
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Cited by 14 •
2012
Described here is a technique in which lipopolysaccharide is injected into the lactating mouse mammary gland via the nipple to simulate mastitis, a condition commonly caused by bacterial infection. Lipopolysaccharide injection results in increased nuclear factor kappa B (NF-κB) signaling, visualized through bioluminescent imaging of an NF-κB luciferase reporter mouse.
06:12
LPS and ATP-induced Death of PMA-differentiated THP-1 Macrophages and its Validation
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Cited by 1 •
2024
This protocol is based on LPS and ATP-induced death of PMA-differentiated THP-1 macrophages. We use flow cytometry to analyze Annexin V and 7-AAD double staining to detect cell death, using the whole cell and employing scanning electron microscopy to observe the cell membrane morphology.
14:48
Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury
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Cited by 3 •
2021
Combined ozone and bacterial endotoxin exposed mice show wide-spread cell death, including that of neutrophils. We observed cellular adaptations such as disruption of cytoskeletal lamellipodia, increased cellular expression of complex V ATP synthase subunit β and angiostatin in broncho-alveolar lavage, suppression of the lung immune response and delayed neutrophil recruitment.
06:43
Increased Recovery Time and Decreased LPS Administration to Study the Vagus Nerve Stimulation Mechanisms in Limited Inflammatory Responses
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Cited by 6 •
2017
Vagus nerve stimulation has proven to have a strong efficacy for decreasing peripheral inflammation. Here, we present a modified vagus nerve stimulation protocol that allows for further examinations of the cholinergic anti-inflammatory mechanisms in limited inflammatory responses.
02:40
Inactivation of Bacteriophages Using Exogenous Lipopolysaccharide to Generate Phage-Free Salmonella Cultures
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2026
Source: Fernández-Fernández, R., et. al. Bacteriophage Removal from Infected Salmonella Cultures. J. Vis. Exp. (2024)This video demonstrates the use of exogenous LPS (Lipopolysaccharide) as a receptor mimic to inactivate lytic bacteriophages in Salmonella cultures. Through sequential incubation, centrifugation, and washing, phages are eliminated, resulting in a viable, phage-free bacterial suspension for downstream applications.
FAQs
The treatment engages two distinct innate immune sensing routes at once: TLR4 responds to the LPS component, while endosomal TLR9 responds to ODN1826. Because these receptors detect different bacterial-associated signals, the combined exposure can reveal how separate inputs coordinate inflammatory gene expression rather than showing the effect of only one recognition pathway.
Separate and combined conditions help distinguish ligand-specific effects from responses shared by both pathways. Measurements of cytokine production, inflammatory transcription, and cellular activation can show whether the combined treatment produces a coordinated response that differs from either stimulus alone. This comparison is useful when determining how multiple bacterial-associated signals interact in the same experimental system.
Convergence on NF-κB and related signaling networks provides a mechanistic link between receptor engagement and changes in inflammatory gene expression. Studying this convergence helps researchers examine how distinct innate immune inputs produce overlapping transcriptional responses, while still preserving information about the separate sensing routes that initiated signaling. The approach therefore connects receptor activity with measurable cellular outcomes.
Genetic perturbations can be evaluated by examining whether they alter cytokine production, inflammatory gene expression, or cellular activation after combined stimulation. Differences between perturbed and unperturbed systems can indicate that a gene influences receptor-linked signaling, pathway convergence, or downstream transcriptional responses. This makes the treatment useful for connecting genotype changes with functional innate immune phenotypes.
A high-level workflow applies LPS and ODN1826 together to an experimental cellular system, then measures outcomes such as cytokine production, inflammatory gene expression, and cellular activation. Researchers can interpret these measurements in relation to TLR4 and TLR9 signaling and compare responses across genetic or treatment conditions. The specific readout depends on the biological question being tested.
The combined stimulus is appropriate when a study asks whether an immune-modulating compound changes a coordinated response to bacterial components. Researchers can assess whether treatment alters cytokine production, inflammatory transcription, or cellular activation under the same stimulation condition. This application helps characterize compounds according to their effects on innate immune signaling rather than on an unstimulated baseline alone.
The model can provide functional measurements of cytokine production, inflammatory gene expression, and cellular activation after coordinated innate immune stimulation. In genetics research, these outcomes help test how perturbations influence immune signaling and transcriptional responses. In immunology, they support analysis of responses to bacterial components and the evaluation of compounds that modify those responses.