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Парные Рост конкуренции Анализ для определения репликации Фитнес человека вируса иммунодефицита
Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
JoVE Journal
Иммунология и инфекции
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JoVE Journal Иммунология и инфекции
Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Парные Рост конкуренции Анализ для определения репликации Фитнес человека вируса иммунодефицита

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11:19 min

May 04, 2015

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11:19 min
May 04, 2015

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Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.

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